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Series GSE87718 Query DataSets for GSE87718
Status Public on May 23, 2018
Title miRNA expression profiles in Dicer WT and KO bone marrow-derived macrophages
Organism Mus musculus
Experiment type Expression profiling by RT-PCR
Summary The global change of the miRNA expression profile during atherosclerosis is due to the infiltration of different types of leukocytes into the arterial vessel wall in addition to disease-specific regulation in vascular cells. Monocyte-derived macrophage accumulation in the subintimal region is critical in the formation of atherosclerotic plaques. However, the role of Dicer, the key enzyme for miRNA biogenesis, during the development of atherosclerosis is currently unknown. To detect the effect of Dicer on miRNA expression in macrophages, the comparison of the miRNA expression profiles was performed in bone marrow-derived macrophages (BMDMs) from LysM-Cre/Dicerflox/flox/Apoe–/– and LysM-Cre/Dicerwt/wt/Apoe–/– mice. This screening combined with miRNA profiling in atherosclerotic aortas may help to identify the crucial miRNAs that play a role in the macrophage function during atherogenesis.
 
Overall design Bone marrow cells were harvested from femora of 6-8 week old male LysM-Cre/Dicerflox/flox/Apoe–/– or LysM-Cre/Dicerwt/wt/Apoe–/– mice (3 mice per group), re-suspended in DMEM-F12/10% FCS/10% L929-conditioned medium, and cultured for 7 days to differentiate into primary macrophages. Total RNA was isolated using mirVana microRNA Isolation Kit.
 
Contributor(s) Schober A, Wei Y
Citation(s) 29748186
Submission date Oct 06, 2016
Last update date May 23, 2018
Contact name Andreas Schober
E-mail(s) aschober@med.lmu.de
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platforms (1)
GPL18082 Applied Biosystems Taqman Array Rodent MicroRNA Cards v3.0
Samples (6)
GSM2339233 Dicer WT BMDMs, Replicate 1
GSM2339234 Dicer WT BMDMs, Replicate 2
GSM2339235 Dicer WT BMDMs, Replicate 3
This SubSeries is part of SuperSeries:
GSE87721 Ago2-RIP-chip and miRNA expression profile in Dicer WT and KO macrophages from bone marrow or aorta
Relations
BioProject PRJNA345625

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87718_Non-normalized_data.txt.gz 9.7 Kb (ftp)(http) TXT
GSE87718_Normalized_data.txt.gz 17.2 Kb (ftp)(http) TXT
GSE87718_fold-change_data.txt.gz 6.1 Kb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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