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| Status |
Public on Apr 01, 2017 |
| Title |
Transcriptomic changes in MYC and PRC2 targets are prominent events in the early pathogenesis of neuroblastoma in TH-MYCN mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
It has been already reported that there are undifferentiated/proliferating neuroblasts in the postnatal sympathetic ganglia in TH-MYCN mice. We established suitable spheroid culture condition that selectively isolates undifferentiated neuroblasts from superior mesenteric ganligon (SMG) of TH-MYCN mice. In the present study, in order to investigate the transcriptomic differences between embryonic day 13.5 (E13.5) WT derived primary spheres and E13.5 TH-MYCN derived passageable spheres, we carried out microarray gene expression analysis. We investigated critical molecular events in MYCN-transformed neuroblastoma cells in TH-MYCN mice.
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| Overall design |
Primary spheres were cultured from E13.5 WT SMG. Passageable spheres were obtained by subculturing spheres from E13.5 TH-MYCN SMG. Four individual biological replicates of E13.5 WT spheres and E13.5 TH-MYCN spheres were subjected to micorarray analyis (two arrays were used for one replicate except for one WT sample).
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| Contributor(s) |
Tsubota S, Kadomatsu K |
| Citation(s) |
28807939 |
| Submission date |
Oct 10, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Shoma Tsubota |
| E-mail(s) |
tsubota@med.nagoya-u.ac.jp
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| Organization name |
Nagoya University Graduate School of Medicine
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| Department |
Department of Molecular Biology
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| Street address |
65 Tsurumai-cho, Showa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE87784 |
Emerging role of polycomb repressive complex 2 in the early neuroblastoma tumorigenesis in TH-MYCN transgenic mice and its association with human neuroblastoma |
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| Relations |
| BioProject |
PRJNA347591 |
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