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Series GSE8790 Query DataSets for GSE8790
Status Public on Mar 23, 2009
Title Comparative analysis of gene expression in A/J CS vs Air lungs.
Organism Mus musculus
Experiment type Expression profiling by array
Summary We hypothesize that gene expression in the CS-exposed lungs of this strain (A/J) of mice would be able to give clues about the molecular mechanism of emphysema development, thus contributing to this phenotype. More specifically, although imbalance in oxidants/antioxidants and proteinase/antiproteinase pathways drives the pathogenesis of COPD, the molecular mechanisms involved in the development of emphysema are poorly understood. In order to test this hypothesis at the gene expression level, we utilized microarray analysis to examine transcriptional differences between CS-exposed and Air-exposed groups of mice.
Keywords: comparative expression profiling
 
Overall design This study utilizes microarray analysis to test these hypotheses. Three sets of lungs were harvested from both groups at each time point (A/J - CS exposed OR Air-exposed: 5 hr, 8 days, 1.5 mos, and 6 mos). RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05.
 
Contributor(s) Rangasamy T, Misra V, Chukwuke U, Zhen L, Tuder R, Biswal S, Tankersley CG
Citation(s) 19286929
Submission date Aug 15, 2007
Last update date Feb 11, 2019
Contact name vikas misra
E-mail(s) vmisra@jhsph.edu
Organization name Johns Hopkins University School of public health
Department Environmental health sciences
Lab Clarke G Tankersley
Street address 615 N. Wolfe street, E6609
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (22)
GSM218335 A/J Air-5hr-exposed-1
GSM218336 A/J Air-5hr-exposed-2
GSM218337 A/J Air-5hr-exposed-3
Relations
BioProject PRJNA102091

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8790_RAW.tar 91.0 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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