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Series GSE8818 Query DataSets for GSE8818
Status Public on Sep 24, 2007
Title Expression changes in intestinal crypts upon deletion of beta-catenin
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Wnt signaling pathway is deregulated in over 90% of human colorectal cancers. β Catenin, the central signal transducer of the Wnt pathway, can directly modulate gene expression by interacting with transcription factors of the TCF/LEF-family. In the present study we investigate the role of Wnt signaling in the homeostasis of intestinal epithelium using tissue-specific, inducible beta-catenin gene ablation in adult mice. Block of Wnt/beta-catenin signaling resulted in rapid loss of transient-amplifying cells and crypt structures. Importantly, intestinal stem cells were induced to terminally differentiate upon deletion of beta-catenin resulting in a complete block of intestinal homeostasis and fatal loss of intestinal function. Transcriptional profiling of mutant crypt mRNA isolated by laser capture micro dissection confirmed those observations and allowed to identify genes potentially responsible for the functional preservation of intestinal stem cells.
Keywords: genetic modification
 
Overall design laser capture microdissection of intestinal crypts, control vs. beta-catenin mutant (2days after induction of deletion by tamoxifen), two rounds of amplification of mRNA
 
Contributor(s) Fevr T, Parisi F, Naef F, Huelsken J
Citation(s) 17785439
Submission date Aug 20, 2007
Last update date Feb 11, 2019
Contact name Joerg Huelsken
Organization name ISREC
Street address Ch. des Boveresses 155
City Epalinges
ZIP/Postal code 1066
Country Switzerland
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM219114 control_crypt_day2_sample1
GSM219115 control_crypt_day2_sample2
GSM219116 mutant_crypt_day2_sample1
Relations
BioProject PRJNA102137

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8818_RAW.tar 66.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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