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Series GSE8835 Query DataSets for GSE8835
Status Public on Aug 22, 2007
Title Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To examine the impact of tumors on the immune system, we compared global gene expression profiles of peripheral blood T cells from previously untreated patients with B cell chronic lymphocytic leukemia (CLL) with those from age-matched healthy donors. Although the cells analyzed were not part of the malignant clone, analysis revealed differentially expressed genes, mainly involved in cell differentiation in CD4 cells and defects in cytoskeleton formation, vesicle trafficking, and cytotoxicity in CD8 cells of the CLL patients. In coculture experiments using CLL cells and T cells from healthy allogeneic donors, similar defects developed in both CD4 and CD8 cells. These changes were induced only with direct contact and were not cytokine mediated. Identification of the specific pathways perturbed in the T cells of cancer-bearing patients will allow us to assess steps to repair these defects, which will likely be required to enhance antitumor immunity.
Gene expression profiling was performed to determine whether CLL cells induce changes in T cells in patients with CLL.
Keywords: comparative gene expression profiling analysis.
 
Overall design CD4 T cells and CD8 T cells were obtained from peripheral blood mononuclear cells of previously untreated patients with CLL and healthy individuals. Gene expression profiling was performed using total RNA and the data were analysed to compare gene expression profile of T cells from patients with CLL to healthy individuals .
 
Contributor(s) Görgün G, Holderried TA, Zahrieh D, Neuberg D, Gribben JG
Citation(s) 15965501
Submission date Aug 21, 2007
Last update date Aug 10, 2018
Contact name Gullu Gorgun
E-mail(s) gullu_gorgun@dfci.harvard.edu
Phone 617 632 3358
Fax 617 632 3222
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (66)
GSM219456 CLL1-CD4
GSM219457 CLL2-CD4
GSM219458 CLL3-CD4
Relations
BioProject PRJNA102171

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8835_RAW.tar 237.1 Mb (http)(custom) TAR (of CEL, TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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