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| Status |
Public on Aug 20, 2008 |
| Title |
Transcriptional regulation by the novel Rho GTPase RhoBTB2. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RhoBTB2 is a novel Rho GTPase that undergoes loss, underexpression and mutation in breast and lung cancer. We have shown that we can mimic loss of RhoBTB2 through siRNA treatment of primary cells. We propose to perform comparative microarray analysis of primary lung cells to establish the identification of the gene targets of RhoBTb2 regulation.
Keywords: Effects of siRNA expression
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| Overall design |
Primary lung cells (NHBE) transfected with siRNA oligonucleotides against cyclophilin or Lamin A/C (controls) were compared to cells transfected with siRNA oligonucleotides against RhoBTB2. We used two siRNA oligonucleotides against RhoBTB2 (1 and 2) to determine any non-specific effects. Cells expressed the siRNA oligonucleotides for 72 hours before total RNA extraction. 10 samples have been analysed.
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| Contributor(s) |
McKinnon CM, Lygoe KA, Skelton L, Mitter R, Mellor H |
| Citation(s) |
18762809 |
| Submission date |
Aug 21, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Harry Mellor |
| URL |
http://0-www-bris-ac-uk.brum.beds.ac.uk/biochemistry/
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| Organization name |
University of Bristol
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| Department |
Department of Biochemistry
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| Street address |
School of Medical Sciences, University Walk
|
| City |
Bristol |
| State/province |
Avon |
| ZIP/Postal code |
BS8 1TD |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA102175 |
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