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Series GSE8856 Query DataSets for GSE8856
Status Public on Mar 31, 2008
Title Sexual dimorphism in the zebrafish hepatic transcriptome and response to dietary carbohydrate
Organism Danio rerio
Experiment type Expression profiling by array
Summary The liver plays a central role in vertebrate glucose homeostasis, and is also one of the most sexually dimorphic organs in terms of gene expression. While the extent of hepatic sexual dimorphism has been well described in mammals, little is known regarding this phenomenon in non-mammalian species, particularly fish. In this study, we examined hepatic gene expression and physiological phenotypes (growth, proximate body composition, retention efficiencies) to determine whether male and female zebrafish respond differently to diets comprised of 0, 15, 25, or 35 % carbohydrate. Using both Affymetrix microarrays and qRTPCR, we observed substantial sexual dimorphism in the hepatic transcriptome, and the response of some genes to dietary carbohydrate manipulation also varied by sex. Males upregulated genes associated with oxidative metabolism, carbohydrate metabolism, energy production, and amelioration of oxidative stress, while females had higher expression levels of genes associated with translation. Males also expressed elevated levels of hnf4a, a gene thought to be involved in regulating hepatic sexual dimorphism in the rodent. Dietary carbohydrate affected hepatic gene expression, growth performance, retention efficiencies of protein and energy, and percentage of moisture, lipid, and ash. Significant diet effects reflected differences between the 0% carbohydrate diet and the other diets, consistent with previous work on other cyprinids showing a high tolerance for dietary carbohydrate. Our data support the use of the zebrafish as a model for the study of both normal and disease states associated with carbohydrate metabolism, and highlight the importance of accounting for both sex and diet
Keywords: Nutritional manipulation, sex comparison
Overall design 17 samples are analyzed from a factorial combination of sex (male or female) and dietary treatment (0% or 25% dietary carbohydrate). 5 biological replicates for females fed each diet, and 3 or 4 biological replicates for males fed the 0% and 25% diets, respectively.
Contributor(s) Robison BD, Settles ML
Citation(s) 20483215
Submission date Aug 23, 2007
Last update date Jan 25, 2018
Contact name Matt L Settles
Phone 5097545396
Organization name University of California, Davis
Department Genome Center
Lab Bioinformatics Core
Street address 451 Health Sciences Sr
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (17)
GSM220169 Liver 25% carb Female fish3
GSM220170 Liver 0% carb Female fish7
GSM220171 Liver 0% carb Female fish8
BioProject PRJNA102211

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Supplementary file Size Download File type/resource
GSE8856_RAW.tar 31.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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