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Series GSE8863 Query DataSets for GSE8863
Status Public on Jun 27, 2008
Title Mouse mammary tumor-initiating cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Using a syngeneic p53 null mouse mammary gland tumor model that closely mimics human breast cancer, we have identified by limiting dilution transplantation as well as in vitro mammosphere and clonogenic assays a Lin-CD29HighCD24High subpopulation of tumor-initiating cells. Differentially expressed genes in the Lin-CD29HighCD24High mouse mammary gland tumor-initiating cell population include those involved in DNA damage response and repair, as well as genes involved in epigenetic regulation previously shown to be critical for stem cell self-renewal.
Keywords: tumor-initiating cells
Overall design The cells were labeled (for FACS sorting) with CD29 and CD24 antibodies, and 4 subpopulations were collected (CD29HighCD24High, CD29HighCD24Low,CD29LowCD24High, and CD29LowCD24Low) to do either in vivo transplantation or to isolate RNA from each of them for array to correlate the in vivo data.

12 samples (RNA of 4 subpopulations based on expression of CD29 and CD24 for each tumor of 3) were included in the identification of differentially expressed genes of tumor-initiating cells. 5 samples (RNA of subpopulations based on expression of CD29 and CD24 of normal mammary epithelial cells ) were included in the normal group analysis. Reference RNA was used to normalize all samples.
Contributor(s) Zhang M
Citation(s) 18559513, 25735774
Submission date Aug 23, 2007
Last update date Feb 11, 2019
Contact name Mei Zhang
Organization name Baylor College of Medicine
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM224616 T2_TG1_CD29HCD24H
GSM224617 T1_TG1_CD29LCD24H
GSM224618 T1_TG1_CD29HCD24H
BioProject PRJNA102225

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Supplementary file Size Download File type/resource
GSE8863_RAW.tar 70.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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