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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 24, 2007 |
| Title |
Comparison of splenic and small intestine lamina propria macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The intestinal immune system must elicit robust immunity against harmful pathogens but restrain immune responses directed against commensal microbes and dietary antigens. The mechanisms that maintain this dichotomy are poorly understood. Here we describe a population of CD11b+F4/80+CD11c– macrophages in the lamina propria (LP) that express several anti-inflammatory molecules including interleukin 10 (IL-10), but little or no pro-inflammatory cytokines, even upon stimulation with Toll-like receptor (TLR) ligands. These macrophages induced, in a manner dependent on IL-10, retinoic acid and exogenous transforming growth factor-β, differentiation of FoxP3+ regulatory T cells. In contrast, LP CD11b+ dendritic cells elicited IL-17 production. This IL-17 production was suppressed by LP macrophages, indicating that a dynamic interplay between these subsets may influence the balance between immune activation and tolerance.
Splenic or small intestine lamina propria CD11b+11c- cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine the unique genetic profile of small intestine lamina propria CD11b+11c- cells.
Keywords: cell type comparison
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| Overall design |
4 samples analyzed, 2 spleen and 2 intestine
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| Contributor(s) |
Pulendran B, Denning T |
| Citation(s) |
17873879 |
| Submission date |
Aug 24, 2007 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Tim Denning |
| Organization name |
La Jolla Institute for Allergy and Immunology
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| Street address |
9420 Athena Circle
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA102235 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8868_RAW.tar |
15.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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