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Series GSE8868 Query DataSets for GSE8868
Status Public on Aug 24, 2007
Title Comparison of splenic and small intestine lamina propria macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary The intestinal immune system must elicit robust immunity against harmful pathogens but restrain immune responses directed against commensal microbes and dietary antigens. The mechanisms that maintain this dichotomy are poorly understood. Here we describe a population of CD11b+F4/80+CD11c– macrophages in the lamina propria (LP) that express several anti-inflammatory molecules including interleukin 10 (IL-10), but little or no pro-inflammatory cytokines, even upon stimulation with Toll-like receptor (TLR) ligands. These macrophages induced, in a manner dependent on IL-10, retinoic acid and exogenous transforming growth factor-β, differentiation of FoxP3+ regulatory T cells. In contrast, LP CD11b+ dendritic cells elicited IL-17 production. This IL-17 production was suppressed by LP macrophages, indicating that a dynamic interplay between these subsets may influence the balance between immune activation and tolerance.
Splenic or small intestine lamina propria CD11b+11c- cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine the unique genetic profile of small intestine lamina propria CD11b+11c- cells.
Keywords: cell type comparison
Overall design 4 samples analyzed, 2 spleen and 2 intestine
Contributor(s) Pulendran B, Denning T
Citation(s) 17873879
Submission date Aug 24, 2007
Last update date Aug 02, 2019
Contact name Tim Denning
Organization name La Jolla Institute for Allergy and Immunology
Street address 9420 Athena Circle
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM224733 Spleen CD11b(+)11c(-)_BP03
GSM224734 Intestine CD11b(+)11c(-)_BP04
GSM224735 Spleen CD11b(+)11c(-)_BP20
BioProject PRJNA102235

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Supplementary file Size Download File type/resource
GSE8868_RAW.tar 15.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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