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Series GSE887 Query DataSets for GSE887
Status Public on Jan 02, 2004
Title Mus musculus cornea experiment
Organism Mus musculus
Experiment type Expression profiling by SAGE
Summary PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea.
Keywords: other
 
 
Contributor(s) Norman BL, Davis JA, Piatigorsky JP
Citation(s) 14744882
Submission date Dec 11, 2003
Last update date Mar 02, 2012
Contact name Barbara Norman
E-mail(s) norman@helix.nih.gov
Phone 301-402-4342
Fax 301-402-0781
Organization name National Institutes of Health
Department National Eye Institute
Lab Laboratory of Molecular & Developmental Biology
Street address MSC2730, 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL11 SAGE:10:NlaIII:Mus musculus
Samples (2)
GSM13195 6-week mouse total cornea
GSM13196 post natal day 9 mouse total cornea
Relations
BioProject PRJNA87893

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