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| Status |
Public on Oct 18, 2016 |
| Title |
Novel metastatic models of human esophageal adenocarcinoma highlight the importance of E-cadherin in cancer metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the identification of FLO-1, and derivation of its isogenic companion FLO-1LM, as two spontaneously metastatic cell line models of human esophageal adenocarcinoma (EAC). We performed genome-wide RNAseq comparing FLO-1LM and FLO-1 parental cells to gain molecular insights into the phenotypic differences between these two cell lines. Using an adjusted p-value<0.05, a total of 375 (307 downregulated, 68 upregulated) genes were differentially regulated in FLO-1LM compared with parental cells. Genome-wide RNAseq profiling revealed significant enrichment of metastasis-related pathways in FLO-1LM cells, many of which are also evident in patient samples. Moreover, CDH1, which encodes the adhesion molecule E-cadherin, was the most significantly downregulated gene in FLO-1LM compared to FLO-1.
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| Overall design |
Genome-wide RNAseq comparing FLO-1LM and FLO-1 parental cells
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| Contributor(s) |
Liu DS, Hoefnagel SJ, Fisher OM, Krishnadath KK, Montgomery KG, Busuttil R, Colebatch A, Read M, Duong CP, Philips WA, Clemons NJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 17, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sanne Hoefnagel |
| Organization name |
Academic Medical Centre Amsterdam
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| Department |
Gastroenterology
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| Lab |
CEMM Krishnadath
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| Street address |
Meibergdreef 9
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| City |
Amsterdam |
| State/province |
Noord-Holland |
| ZIP/Postal code |
1105AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA348681 |
| SRA |
SRP091645 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE88802_RAW.tar |
850.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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