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| Status |
Public on Sep 05, 2007 |
| Title |
Comparative gene expression profiles of T-dependent and T-independent germinal centre B cells in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Selection of B cells subjected to hypermutation in germinal centres (GC) during T-dependent (TD) antibody responses yields memory cells and long-lived plasma cells that produce high affinity antibodies biased to foreign antigens rather than self-antigens. GC also form in T-independent (TI) responses to polysaccharide antigens but failed selection results in GC involution and memory cells are not generated. To date there are no markers that allow phenotypic distinction of T-dependent and T-independent germinal centre B cells. We have now compared the global gene expression of GC B cells purified from mice immunized with either TD or TI antigens and identified eighty genes that are differentially expressed in TD GC.
Keywords: T-dependent and T-independent germinal center B cells
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| Overall design |
QMxB6 F1 mice were immunised with T-independent antigens (NP-Ficoll) or T-dependent antigens (NP-CGG). T-dependent responses were analysed in carrier primed mice and non-carrier primed mice. The kinetics in carrier primed mice are comparable to those of TI-2 antigens, and thus, both of these groups were analysed 4 days after immunization with soluble antigen. The response in non-carrier primed mice peaks later, and was therefore analysed on day 10 after immunization. Germinal centre B cells were MACS enriched and then FACS sorted according to GL-7 and B220 expression. Sorted B cells from the relevant mice were pooled (4-10 mice per sample). Each sample thus contains B cells from different mice, and the replicates are biological, not technical replicates.
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| Contributor(s) |
Vinuesa CG, Yu D, Silva DG, Goodnow CG, Cooke MP |
| Citation(s) |
17938642 |
| Submission date |
Aug 29, 2007 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Diego G Silva |
| E-mail(s) |
diego.silva@anu.edu.au
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| Organization name |
John Curtin School of Medical Research
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| Department |
Division of Immunology and Genetics
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| Lab |
Immunogenomics Laboratory
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| Street address |
Mills Road
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| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (8)
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| GSM225630 |
Resting B cells (pooled from 6 mice) 1 |
| GSM225631 |
Resting B cells (pooled from 9 mice) 2 |
| GSM225632 |
Day 4 T-dependent GC B cells in carrier primed mice (pooled from 6 mice) 1 |
| GSM225633 |
Day 4 T-dependent GC B cells in carrier primed mice (pooled from 9 mice) 2 |
| GSM225634 |
Day 10 T-dependent GC B cells in non-carrier-primed mice (pooled from 6 mice) 1 |
| GSM225635 |
Day 10 T-dependent GC B cells in non-carrier-primed mice (pooled from 6 mice) 2 |
| GSM225636 |
Day 4 T-independent GC B cells (pooled from 4 mice) 1 |
| GSM225637 |
Day 4 T-independent GC B cells (pooled from 10 mice) 2 |
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| Relations |
| BioProject |
PRJNA102299 |
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