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| Status |
Public on Jul 12, 2017 |
| Title |
Deregulation of lncRNAs by Kaposi's sarcoma-associated herpesvirus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Deregulation of host lncRNAs following infection with Kaposi’s sarcoma-associated herpesvirus (KSHV) was assessed. Total RNA was prepared from uninfected TIVE cells (telomerase-immortalized vein endothelial cells), and from TIVE cells latently infected with either wild-type KSHV or with a deletion mutant (Δcluster-KSHV) lacking ten of the twelve KSHV microRNA genes. The RNA was converted to labeled cRNA and used to probe a human lncRNA microarray. The goal was to investigate which host lncRNAs were either up- or down-regulated during latent infection with KSHV, and to determine if deregulation of host lncRNAs was dependent on KSHV miRNAs.
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| Overall design |
One biological replicate of each of mock infected, wtKSHV-infected and Δcluster-KSHV infected cells was used. Three technical replicates were performed for each.
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| Contributor(s) |
Sethuraman S, Gay LA, Jain V, Haecker I, Renne R |
| Citation(s) |
28715488 |
| Submission date |
Oct 24, 2016 |
| Last update date |
Mar 12, 2018 |
| Contact name |
Rolf F Renne |
| E-mail(s) |
rrenne@ufl.edu
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics & Microbiology
|
| Lab |
CGRC 375
|
| Street address |
2033 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL16956 |
Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA350313 |
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