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Series GSE89120 Query DataSets for GSE89120
Status Public on Oct 17, 2017
Title Evidence of stress in beta cells obtained with laser capture microdisssection from pancreases of brain-dead donor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain dead, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during culture tissue. Isolated islets used for transplantation are known to be stressed, which can result from the circumstances of death, in particular brain death, the preservation of the pancreas with its warm and cold ischemia, from the trauma of the isolation process, and the complex events that occur during tissue culture. The current study focused upon the events that occur before the islet isolation procedure. Pancreases were obtained from brain dead donors (n=7) with mean age 50 (11) and normal pancreatic tissue obtained at surgery done for pancreatic neoplasms (n=7), age 69 (9). Frozen sections were subjected to laser capture microdissection (LCM) to obtain beta-cell rich islet tissue, from which extracted RNA was analyzed with microarrays. Gene expression of the two groups was evaluated with differential expression analysis for genes and pathways. Marked changes were found in pathways concerned with endoplasmic reticulum stress with its unfolded protein response (UPR), apoptotic pathways and components of inflammation. In addition, there were changes is genes important for islet cell identify. These findings advance our understanding of why islets are stressed prior to transplantation, which may lead to strategies to reduce this stress and lead to better clinical outcomes.
 
Overall design beta-cell Enrichment with LCM and Global Gene Expression Profiling Using Affymetrix Microarray
 
Contributor(s) Weir GC, Ebrahimi A
Citation(s) 28252345
Submission date Oct 24, 2016
Last update date Oct 20, 2017
Contact name Hui Pan
E-mail(s) Hui.Pan@joslin.harvard.edu
Organization name Joslin Diabetes Center
Department Bioinformatics and Biostatistics Core
Street address 1 Joslin Place
City Boston
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL1352 [U133_X3P] Affymetrix Human X3P Array
Samples (14)
GSM2359114 Surgical1
GSM2359115 Surgical2
GSM2359116 Cadaver1
Relations
BioProject PRJNA350321

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Supplementary file Size Download File type/resource
GSE89120_RAW.tar 59.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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