|
| Status |
Public on Oct 26, 2016 |
| Title |
RBM25 is a global splicing factor promoting inclusion of alternatively spliced exons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to determine the role of RBM25 in pre-mRNA splicing and examine whether lysine 77 contributes to this function.
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| |
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| Overall design |
We examined mRNA transcript level and splicing events in 293T cells in two experiments. First is CRISPR/Cas9 depletion of RBM25 vs control sgRNA. Second is over-expression of GFP, RBM25 or RBM25 K77R mutant.
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| |
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| Contributor(s) |
Carlson SM, Soulette CM, Yang Z, Elias JE, Brooks AM, Gozani O |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Or Gozani |
| E-mail(s) |
ogozani@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Gozani
|
| Street address |
371 Serra Mall
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (10)
|
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| Relations |
| BioProject |
PRJNA350387 |
| SRA |
SRP092031 |
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