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| Status |
Public on Oct 26, 2016 |
| Title |
Comparison of gene expression changes following knockdown of KMT2A and MSK1 in mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The KMT2A/MLL1 lysine methyltransferase complex is an epigenetic regulator of selected developmental genes, in part through the SET-domain-catalyzed methylation of H3K4. It is essential for normal embryonic development and haematopoiesis and frequently mutated in cancer. The catalytic properties and targeting of KMT2A/MLL1 depend on the proteins with which it complexes and the post-translational protein modifications which some of these proteins put in place, though detailed mechanisms remain unclear. We have shown that KMT2A/MLL1 (both native and FLAG-tagged) and Msk1 (RPS6KA5), co-immunoprecipitated in various cell types.
This experiment showed that the great majority of genes whose activity changed on KTM2A/MLL1 knockdown responded comparably to Msk1 knockdown.
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| Overall design |
A 10 array study in which KMT2A (Mll1) or MSK1 were knocked down in mouse embryonic fibroblasts. There were 3 control (mock transfected) samples, 4 KMT2A shRNA samples and 3 MSK1 shRNA samples.
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| Contributor(s) |
Halsall JA, Wiersma M, Turan N, Turner BM, Nightingale KP |
| Citation(s) |
27895715 |
| Submission date |
Oct 25, 2016 |
| Last update date |
Feb 09, 2017 |
| Contact name |
John Halsall |
| E-mail(s) |
j.halsall@bham.ac.uk
|
| Organization name |
University of Birmingham
|
| Department |
Cancer and Genomic Sciences
|
| Lab |
Chromatin & Gene Expression Group
|
| Street address |
Medical School, Vincent Drive
|
| City |
Birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL10192 |
NimbleGen Mus musculus MM9 Expression Array (12x135k) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA350585 |
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