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| Status |
Public on Apr 03, 2008 |
| Title |
Effect of loss of CREB and CREM on kainate-induced gene expression in the hippocampus (MG-U74B). |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Ablation of the Creb1 gene in forebrain neurons was performed using the Cre/loxP system, with the recombinase expressed from the Camk2alfa promoter. Mice were crossed into the Crem KO background to prevent compensation of CREB loss by CREM overexpression.
Our goal was to analyze how loss of CREB will affect acitivity-regulated transcription induced by strong stimulation, i.e. kainate.
Keywords: Treatment x Genotype
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| Overall design |
Animals were injected i.p. with either 20 mg/kg kainate or saline and sacrificed after one hour by cervical dislocation. Expression profiling was performed using total RNA isolated from the hippocampus.
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| Contributor(s) |
Rodriguez-Parkitna J, Lemberger T |
| Citation(s) |
18424767 |
| Submission date |
Sep 04, 2007 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Jan Rodriguez Parkitna |
| E-mail(s) |
j.rodriguez@dkfz.de
|
| Phone |
+49 6221 423437
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| Organization name |
German Cancer Research Center
|
| Lab |
Molecular Biology of the Cell I
|
| Street address |
Im Neuenheimer Feld 581
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
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| Samples (12)
|
| GSM226783 |
Hippocampi from saline treated control animal, biological rep. 1 |
| GSM226784 |
Hippocampi from saline treated control animal, biological rep. 2 |
| GSM226785 |
Hippocampi from saline treated control animal, biological rep. 3 |
| GSM226786 |
Hippocampi from kainate treated control animal, biological rep. 1 |
| GSM226787 |
Hippocampi from kainate treated control animal, biological rep. 2 |
| GSM226788 |
Hippocampi from kainate treated control animal, biological rep. 3 |
| GSM226789 |
Hippocampi from saline treated mutant animal, biological rep. 1 |
| GSM226790 |
Hippocampi from saline treated mutant animal, biological rep. 2 |
| GSM226791 |
Hippocampi from saline treated mutant animal, biological rep. 3 |
| GSM226792 |
Hippocampi from kainate treated mutant animal, biological rep. 1 |
| GSM226793 |
Hippocampi from kainate treated mutant animal, biological rep. 2 |
| GSM226794 |
Hippocampi from kainate treated mutant animal, biological rep. 3 |
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| Relations |
| BioProject |
PRJNA102373 |
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