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| Status |
Public on Jun 13, 2017 |
| Title |
Transcriptomic analysis of endometrial tissues following GnRH agonist treatment in a mouse adenomyosis model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: We sought a transcriptomic analysis of endometrial tissues following GnRH agonist treatment in a mouse model
Methods: The neonatal female mice were randomly divided into three groups: control group, adenomyosis group and GnRH agonist treatment group. The pregnant outcome was observed and compared among the three groups. Besides, endometrial tissues from Day 4 of pregnancy were transcriptomic analysed using bioinformatics approaches.
Results:We found that the litter size was smaller in adenomyosis group than control group (8±0.28vs. 11±0.26; P＜0.05).However, the average live litter size was increased (10±0.28 vs.8±0.28; P＜0.05) after GnRH agonist treatment. Transcriptomic analysis showed that compared with the adenomyosis group, 359 genes were differentially expressed in the GnRH agonist treatment group: 218 were down regulated and 141 were up regulated.Gene function analysis showed that the differentially expressed genes were related to diverse biological processes, including estrogen metabolism, cell cycle, and metabolites biosynthesis.
Conclusions: GnRH agonist can improve the pregnancy outcome of adenomyosis in mouse model. Besides pituitary down regulation effect, other possible mechanisms may play role in GnRH agonist treatment, e.g., regulate cell proliferation cycle. It may be useful for the further understand the the mechanisms of the GnRH agonist in adeomyosis treatment.
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| Overall design |
Transcriptomic analysis of endometrial tissues between GnRH agonist treatment group and non-GnRH agonist treatment group in a mouse adenomyosis model
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| Contributor(s) |
Guo S, Lu X, Gu R, Zhang D, Sun Y, Feng Y |
| Citation(s) |
28331289 |
| Submission date |
Nov 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
SONG GUO |
| E-mail(s) |
304288026@qq.com
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| Phone |
+86-21-6437-4262
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| Organization name |
Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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| Street address |
RUIJIN 2TH ROAD
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| City |
SHANGHAI |
| ZIP/Postal code |
20025 |
| Country |
China |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA352265 |
| SRA |
SRP092485 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89463_All_Counts.txt.gz |
397.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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