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Series GSE8949 Query DataSets for GSE8949
Status Public on Dec 12, 2007
Title Gene expression changes in mouse aorta during activation of or interference with PPAR gamma signaling.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Ligand-mediated activation of the nuclear hormone receptor PPAR gamma lowers blood pressure and improves glucose tolerance in humans. Two naturally occurring mutations (P467L, V290M) in the ligand binding domain of PPAR gamma have been described in humans that lead to severe insulin resistance and hypertension. Experimental evidence suggests that these mutant versions of PPAR gamma act in a dominant negative fashion. To better understand the molecular mechanisms underlying PPAR gamma action in the vasculature, we determined the gene expression patterns in mouse aorta in response to activation or interference with the PPAR gamma signaling pathway.
Keywords: time course, dose response
Overall design To assess the response to PPAR gamma interference, we used adult mice containing a dominant negative form of PPAR gamma. These mice have a targeted P465L mutation, which is equivalent to the P467L mutant, described in human patients. Wild-type littermates were used as the genetic control. The PPAR gamma signaling pathway was activated by administration of rosiglitazone for either 2 or 14 days to adult mice (C57BL/6J strain) at a dose of 3 or 10 mg/kg/day via the food. Control mice were fed standard mouse chow. For the microarray hybridizations, 2-3 biological replicates from each experimental group were used. Biological replicates were RNA pooled from 8 different mouse aortas. All the microarray procedures were conducted at the University of Iowa DNA Core facility using standard Affymetrix protocols. In brief, approximately 3 ug of total RNA was used as input to a one-step amplification procedure to generate biotin-labeled RNA fragments for hybridization to the Affymetrix GeneChip Mouse Genome 430 2.0 array.
Contributor(s) Keen HL, Sigmund CD, Beyer AM, Halabi CM, de Lange WJ, Faraci FM
Citation(s) 20018933, 18316027, 18285614
Submission date Sep 04, 2007
Last update date Apr 01, 2020
Contact name Henry Keen
Phone 319-335-8926
Organization name University of Iowa
Department Pharmacology
Lab Dr. Curt D. Sigmund's Laboratory
Street address 2-370 BSB
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM226823 Mouse Aorta, Control_for_Rosiglitazone_A
GSM226824 Mouse Aorta, Control_for_Rosiglitazone_B
GSM226825 Mouse Aorta, Control_for_Rosiglitazone_C
BioProject PRJNA102379

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Supplementary file Size Download File type/resource
GSE8949_RAW.tar 70.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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