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Series GSE89506 Query DataSets for GSE89506
Status Public on May 18, 2017
Title Blocking promiscuous activation at cryptic promoters directs cell type–specific gene expression
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary This SuperSeries is composed of the SubSeries listed below.

Abstract
To selectively express cell type–specific transcripts during development, it is critical to maintain genes required for other lineages in a silent state. Here, we show in the Drosophila male germline stem cell lineage that a spermatocyte-specific zinc finger protein, Kumgang (Kmg), working with the chromatin remodeler dMi-2 prevents transcription of genes normally expressed only in somatic lineages. By blocking transcription from normally cryptic promoters, Kmg restricts activation by Aly, a component of the testis-meiotic arrest complex, to transcripts for male germ cell differentiation. Our results suggest that as new regions of the genome become open for transcription during terminal differentiation, blocking the action of a promiscuous activator on cryptic promoters is a critical mechanism for specifying precise gene activation.
 
Overall design Refer to individual Series
Web link http://science.sciencemag.org/content/356/6339/717
 
Citation(s) 28522526
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 GM061986 Regulation of Cell-Type Specific Transcription in Spermatocytes STANFORD UNIVERSITY MARGARET T FULLER
Submission date Nov 03, 2016
Last update date May 15, 2019
Contact name Robert E Kingston
E-mail(s) kingston@molbio.mgh.harvard.edu
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Kingston lab.
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (2)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (52)
GSM1679137 AdultTestes_UAS-RNAi-kmg (VDRC# 107395); TM6b_rep1
GSM1679138 AdultTestes_UAS-RNAi-kmg (VDRC# 107395); TM6b_rep2
GSM1679139 AdultTestes_UAS-RNAi-kmg (VDRC# 107395); bam-gal4, UAS-dicer2_rep1
This SuperSeries is composed of the following SubSeries:
GSE68696 Gene expression of fly testes with dMi-2, kumgang (CG5204) knock downs
GSE89380 Gene expression of fly testes with dMi-2, kumgang (CG5204) knock downs and wild-type heads by RNA-Seq
GSE89381 ChIP-Seq with Kumgang (Kmg), dMi-2, and Aly from wild-type and kmg KD Drosophila melanogaster testes
Relations
BioProject PRJNA352401

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89506_RAW.tar 5.5 Gb (http)(custom) TAR (of BW, CEL)
SRA Run SelectorHelp
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