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Series GSE89597 Query DataSets for GSE89597
Status Public on Jul 13, 2017
Title Splicing activation by Rbfox requires self-aggregation through its tyrosine-rich domain
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To determine how the higher order assembly of Rbfox proteins affect Rbfox-dependent splicing regulation, we expressed Rbfox wildtype and its mutant protein in Flp-In™ T-REx™ 293 Rbfox2-/- cells and extracted RNA from these cells to perform RASL-seq which profiles thousands of alternative splicing event.
 
Overall design Flp-In™ T-REx™ 293 Cell Line (in triplicate) were transfected by Rbfox1 wildtype and its mutants, followed by Dox induction. Total RNAs were extracted and been analyzed by RASL-seq.
 
Contributor(s) Ying Y, Black DL
Citation(s) 28708999
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 GM114463 Mechanisms of Alternative Splicing Regulation by Rbfox Proteins UNIVERSITY OF CALIFORNIA LOS ANGELES Douglas L. Black
Submission date Nov 07, 2016
Last update date May 15, 2019
Contact name Douglas L. Black
E-mail(s) dougb@microbio.ucla.edu
Organization name UCLA
Department MIMG
Lab 6-780 MacDonald Research Laboratories
Street address 675 Charles E. Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (12)
GSM2385208 Ctrl rep1
GSM2385209 Ctrl rep2
GSM2385210 Ctrl rep3
Relations
BioProject PRJNA352682
SRA SRP092755

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89597_RASLseq.csv.gz 203.8 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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