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Series GSE8960 Query DataSets for GSE8960
Status Public on Sep 01, 2008
Title The TLR2/NOD2/RICK signaling axis regulates stimulus-specific IL-10 production
Organism Mus musculus
Experiment type Expression profiling by array
Summary Recognition and response to gram-positive bacteria by macrophages and dendritic cells is mediated in part through TLR2. We found that that Streptococcus pneumoniae cell wall fragments, containing primarily peptidoglycan and teichoic acids, induced prodigious secretion of IL-10 from macrophages and dendritic cells and was dependent on TLR2 and NOD2, a cytoplasmic CARD-NACHT-LRR protein encoded by Card15. IL-10 secretion in response to cell walls was also dependent on RICK/RIP2, a kinase associated with NOD2, and MYD88 but independent of the ERK/p38 pathway. The reduction of IL-10 secretion by cell wall-activated NOD2-deficient myeloid–derived cells translated into downstream effects on IL-10 target gene expression and elevations in subsets of pro-inflammatory cytokine expression normally restrained by autocrine/paracrine effects of IL-10. Since NOD2 is linked to aberrant immune responses in Crohn’s Disease patients bearing mutations in CARD15, the temporal and quantitative effects of the TLR2/NOD/RICK pathway on IL-10 secretion may affect homeostatic control of immune responses to gram-positive bacteria.
Keywords: immune response simulated pathogen response
Overall design KO mice were treated with a cel wall extract and evaluated for immune response 1 hr and 4 hrs after treatment
Contributor(s) Moreira L, El Kasmi KC, Smith AM, Finkelstein D, Fillon S, Tuomanen E, Cheng G, Nunez G, Murray PJ
Citation(s) 18549453
Submission date Sep 05, 2007
Last update date Feb 11, 2019
Contact name David Finkelstein
Phone 9014953931
Organization name St Jude Children's Research Hospital
Department Computational Biology
Street address 332 N. Lauderdale St.
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (18)
GSM226951 Mouse immune cells 1 hr after treatment (mcw054)
GSM226952 Mouse immune cells 1 hr after treatment (mcw051)
GSM226954 Mouse immune cells time zero control (mcw047)
BioProject PRJNA102401

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Supplementary file Size Download File type/resource
GSE8960_RAW.tar 60.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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