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Series GSE9013 Query DataSets for GSE9013
Status Public on Nov 12, 2007
Title Expression data from side-population sorted putative intestinal stem cells.
Organism Mus musculus
Experiment type Expression profiling by array
Summary While the existence of intestinal epithelial stem cells (IESCs) has been well established, their study has been limited due to the inability to isolate them. Previous work has utilized side population (SP) sorting of the murine small intestinal mucosa to isolate a viable fraction of cells enriched for putative IESCs. We have used microarray analyses to characterize the molecular features of this potential stem cell population.
Keywords: Comparitive gene expression analysis
 
Overall design Fluorescence activated cell sorting of cells stained with Hoechst 33342 and FITC labeled anti-CD45 antibody was used to isolate CD45-/SP and CD45-/nSP cell fractions from mucosal specimens pooled from 3 mice. Intact jejunum specimens were collected prior to sorting. 4 sorts were completed. Therefore, 3 experimental groups were defined: CD45-/SP, CD45-/nSP and intact jejunum, each with 4 biological replicates. Total RNA was extracted from each experiment group for each sort, resulting in 12 specimens submitted for analysis using Affymetrix 430 2.0 Gene Chips.
 
Contributor(s) Gulati AS, Ochsner SA, Henning SJ
Citation(s) 18006601
Submission date Sep 11, 2007
Last update date Feb 11, 2019
Contact name Ajay S Gulati
E-mail(s) ajay_gulati@med.unc.edu
Phone (919) 966-7886
Organization name University of North Carolina at Chapel Hill
Department Pediatric Gastroenterology
Street address 130 Mason Farm Road, CD#7220
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM228795 CD45-negative SP cell fraction, biological rep1
GSM228796 CD45-negative nSP cell fraction, biological rep1
GSM228797 Intact jejunum, biological rep1
Relations
BioProject PRJNA102501

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Supplementary file Size Download File type/resource
GSE9013_RAW.tar 73.7 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file
Processed data included within Sample table

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