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Series GSE9044 Query DataSets for GSE9044
Status Public on Sep 19, 2007
Title HOXB4 target genes in ES cell-derived embryoid bodies (EBs)
Organism Mus musculus
Experiment type Expression profiling by array
Summary To unravel the molecular mechanism by which HOXB4 promotes the expansion of early hematopoietic progenitors within differentiating ES cells, we analzed the gene expression profiles of embryoid bodies (EBs) in which transcription of HOXB4 had been induced or not induced. A substantial number of the identified HOXB4 target genes are involved in signaling pathways important for controlling self-renewal, maintenance and differentiation of stem cells. Furthermore, we demonstrate that HOXB4 activity and FGF-signaling are intertwined. HOXB4-mediated expansion of ES cell-derived early progenitors was enhanced by specific and complete inhibition of FGF-receptors. In contrast, the expanding activity of HOXB4 on hematopoietic progenitors in day4-6 embryoid bodies was blunted in the presence of basic FGF (FGF2) indicating a dominant negative effect of FGF-signaling on the earliest hematopoietic cells. Taken together, we show that modulation of FGF signaling is an essential feature of HOXB4 activity in the context of embryonic hematopoiesis.
Keywords: plus/minus induction of HOXB4 gene expression by treatment with doxycycline (Dox)
Overall design The Hoxb4i ES cell line (Kyba et al. 2002, Cell 109:29-37) contains an integrated “tet-on” cassette that allows induction of HOXB4 expression upon treatment with doxycycline. These ES cells can be used to produce hematopoietic cells through the formation of embryoid bodies (EBs). Hematopoiesis starts in these EBs at day 4 and the differentiation into hematopoietic fates can be quantified by colony assays on methyl-cellulose using cells dissociated from EBs at day 6 of incubation. The induction of HOXB4 by incubation with doxycycline increases the production of hematopoietic progenitors within EBs by day 6. Using this specific ES cell line, we compared the transcriptome between embryoid bodies (EBs) in which transcription of HOXB4 had been induced or not induced from day 4 to day 6 (48hours).
Biological replicates: 3
Contributor(s) Mallo M, Santos A, Ribeiro A
Citation(s) 17940039
Submission date Sep 14, 2007
Last update date Feb 11, 2019
Contact name Moises Mallo
Organization name Instituto Gulbenkian de Ciencia
Lab Patterning and Morphogenesis
Street address Rua da Quinta Grande 6
City Oeiras
ZIP/Postal code 2780-156
Country Portugal
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM229799 EBs w/o doxy replica A
GSM229800 EBs with doxy replica A
GSM229801 EBs w/o doxy replica B
BioProject PRJNA102549

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9044_RAW.tar 20.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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