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| Status |
Public on Oct 02, 2017 |
| Title |
Induction of SFTPC+ cells from human induced pluripotent stem cell-derived CPMhigh progenitor cells by forming alveolar organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray analysis was performed on P0 SFTPC+ cells, P0 SFTPC- cells and CPMhigh progenitor cells derived form human induced pluripotent stem cells (B2-3 SFTPC-GFP reporter hiPSCs), respectively.
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| Overall design |
CPMhigh progenitor cells stepwisely induced from hiPSCs were isolated using fluorescence activated cell sorting (FACS) on day 21. Isolated CPMhigh cells were cocultured with human fetal lung fibroblasts forming alveolar organoids (AOs). P0 SFTPC+ cells and P0 SFTPC- cells were isolated from AOs on Day 35.
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| |
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| Contributor(s) |
Yamamoto Y, Gotoh S |
| Citation(s) |
28967890 |
| Submission date |
Nov 28, 2016 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Shimpei Gotoh |
| Organization name |
Kyoto University
|
| Department |
Center for iPS cell Research and Application
|
| Lab |
Gotoh Lab
|
| Street address |
53 Kawaharacho, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA355126 |
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