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| Status |
Public on Sep 18, 2007 |
| Title |
Changes in gene expression profile in mammalian cells upon Raf activation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparison of the changes in the gene expression profile of cells in which there is an activation of the Raf oncogene in the absence of any other extracellular signals
Keywords: Response to oncogenic activation of the MAPK pathway
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| Overall design |
Primary rat Schwann cells containing an inducible RafER allele were plated on poly-L-lysine plus fibronectin coated dishes on DMEM + 3% FCS containing medium. Next day cells were transferred to minimal medium (DMEM containing 100 μg ml-1 transferrin, 100 μg ml-1 bovine serum albumin, 16.1 μg ml-1 putrescine, 39 ng ml-1 selenium) for 24 h. Cells were then treated with either the vehicle (EtOH) or with Tamoxifen for 24 h. Three independent experiments were performed.
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| Contributor(s) |
Echave P, Lloyd AC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 17, 2007 |
| Last update date |
Jul 31, 2017 |
| Contact name |
ALISON C LLOYD |
| E-mail(s) |
alison.lloyd@ucl.ac.uk
|
| Organization name |
UNIVERSITY COLLEGE LONDON
|
| Department |
LMCB
|
| Lab |
LLOYD
|
| Street address |
GOWER STREET
|
| City |
LONDON |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA102575 |
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