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| Status |
Public on Jul 25, 2017 |
| Title |
Mechanisms of transcription factor-mediated direct reprogramming of mouse embryonic stem cells to trophoblast stem-like cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Direct reprogramming can be achieved by forced expression of transcription factors (TFs). Yet how such TFs mediate repression of initial cell-type-specific genes while activating target cell-type-specific genes is unclear. Here, we achieve embryonic stem (ES) to trophoblast stem (TS)-like cell reprogramming by introducing individual TS-specific “CAG” factors (Cdx2, Arid3a, Gata3). We interrogated their chromosomal target occupancies, modulation of global transcriptome and chromatin accessibility at the initial stage of reprograming. Our findings uncovered a sequential, two-step mechanism of cellular reprogramming in which repression of exiting ES pluripotency is followed by activated conversion to TS cells by CAG factors.
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| |
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| Overall design |
TS-like cells were generated from ES cells using CAG factors, followed by deep sequencing, using Illumina
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| |
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| Contributor(s) |
Kim J |
| Citation(s) |
28973471 |
| Submission date |
Dec 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jonghwan Kim |
| E-mail(s) |
jybella@utexas.edu
|
| Phone |
512-232-8046
|
| Organization name |
University of Texas at Austin
|
| Department |
Department of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology
|
| Lab |
Kim Lab
|
| Street address |
2506 Speedway NMS 4.246
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (25)
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| Relations |
| BioProject |
PRJNA355855 |
| SRA |
SRP094427 |
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