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| Status |
Public on Jul 25, 2018 |
| Title |
ORC2 ChIP-seq in Drosophila larval fat body |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We analyzed Origin Recognition Complex Subunit 2 (ORC2) ChIP-seq from hand-dissected fat body tissue from 68hr (after egg laying, AEL), 92hr AEL, and late-third wandering Drosophila melanogaster larvae. Fat body was dissected from wild-type (OrR) males and testes were removed. We examined ORC2 binding genome-wide with particular focus on the underreplicated regions in the fat body.
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| Overall design |
ChIP-seq of ORC2 bound to fat body chromatin from 68hr AEL, 92hr AEL, and late-third wandering wild-type (OrR) Drosophila melanogaster male larvae. Sequences analyzed by Illumina sequencing. Two biological replicates are included for each ChIP reaction.
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| Contributor(s) |
Hua BL, Kashevsky H, Bell GW, Von Stetina JR, Orr-Weaver TL |
| Citation(s) |
30134926 |
| Submission date |
Dec 05, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Terry L. Orr-Weaver |
| E-mail(s) |
weaver@wi.mit.edu
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| Phone |
617-258-5245
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| Organization name |
Whitehead Institute
|
| Street address |
455 Main Street
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| City |
Cambridge |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE90917 |
mRNA-seq and ORC2 ChIP-seq in Drosophila larval fat body |
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| Relations |
| BioProject |
PRJNA356323 |
| SRA |
SRP094592 |
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