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| Status |
Public on Dec 01, 2017 |
| Title |
Differential genomic-methylation profile of U937 cells transduced with WT/mutant-DNMT3A |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
DNA methyltransferase 3A (DNMT3A) gene is mutated in various myeloid neoplasms including acute myeloid leukemia (AML), especially at the Arg882 and associated with inferior outcomes. Despite the current progress of functional role of DNMT3A mutations, the molecular pathogenesis of myeloid malignancies remains poorly understood. The mechanisms of AML transformation and functional role of DNMT3A mutations through its target genes in the leukemogenesis remain to be explored. Here we wished to perform the differential genomic-methylation profile in U937 cells over-expressed with DNMT3A-Arg882His/Cys (R882H/C) mutations including DNMT3A-WT and vector using Illumina MethylationEPIC BeadChip microarray.
Results: Differential genomic-methylation assess identified both hypo- and hypermethylation features in different regions throughout the whole genome of DNMT3A mutants-transduced U937 cells.
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| Overall design |
Genomic methylation profiling of U937 cells with empty vector, or overexpression of wildtype DNMT3A, DNMT3A mutant R882C or DNMT3A mutant R882H.
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| Contributor(s) |
Bera R, Shih L, Chiu M, Huang Y, Liang D, Lee Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 06, 2016 |
| Last update date |
Jan 20, 2019 |
| Contact name |
Yun-Shien Lee |
| E-mail(s) |
bojack@mail.mcu.edu.tw
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| Organization name |
Ming Chuan Univ
|
| Department |
Dept. Biotechnology
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| Lab |
GMRCL
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| Street address |
TauYau
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| City |
TauYau |
| State/province |
Taiwan |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
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| Samples (4)
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| GSM2417753 |
U937 cells transduced with pCDH-MSCV-puro lentivirus vector_methylation |
| GSM2417754 |
U937 cells transduced with pCDH-MSCV-puro-DNMT3A-WT lentivirus vector_methylation |
| GSM2417755 |
U937 cells transduced with pCDH-MSCV-puro-DNMT3A-R882C lentivirus vector_methylation |
| GSM2417756 |
U937 cells transduced with pCDH-MSCV-puro-DNMT3A-R882H lentivirus vector_methylation |
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| This SubSeries is part of SuperSeries: |
| GSE90934 |
Differential gene expression and genomic methylation profile of U937 cells transduced with WT/mutant-DNMT3A |
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| Relations |
| BioProject |
PRJNA356407 |
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