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Series GSE9123 Query DataSets for GSE9123
Status Public on Sep 22, 2007
Title transcription factor PlagL2 regulates steps in chylomicron metabolism
Organism Mus musculus
Experiment type Expression profiling by array
Summary Enterocytes assemble dietary lipids into chylomicron particles that are taken up by intestinal lacteal vessels and peripheral tissues. Although chylomicrons are known to assemble in part within membrane secretory pathways, the modifications required for efficient vascular uptake are unknown. We report that the transcription factor Pleomorphic adenoma gene-like 2 (PLAGL2) is essential for this aspect of dietary lipid metabolism. PlagL2-/- mice die from post-natal wasting owing to failure of fat absorption. Lipids modified in the absence of PlagL2 exit from enterocytes but fail to enter interstitial lacteal vessels. Dysregulation of enterocyte genes closely linked to intracellular membrane transport identified candidate regulators of critical steps in chylomicron assembly. PlagL2 thus regulates essential and poorly understood aspects of dietary lipid absorption and its deficiency represents an authentic animal model with implications for amelioration of obesity or the metabolic syndrome.
Keywords: gene expression profiling analysis
 
Overall design Total RNA was extracted from 4 knockout and 4 wild-type mouse small intestines at 18.5 dpc using the Macherey-Nagel Nucleospin kit. cRNA synthesis and labeling, hybridization to Affymetrix (Santa Clara, CA) MOE430 2.0 expression arrays, and data acquisition occurred on the Affymetrix GeneChip Instrument System.
 
Contributor(s) Van Dyck F, Braem CV, Chen Z, Declercq J, Deckers R, Kim B, Ito S, Wu MK, Cohen DE, Dewerchin M, Derua R, Waelkens E, Fiette L, Roebroek A, Schuit F, Van de Ven W, Shivdasani RA
Citation(s) 17983586
Submission date Sep 20, 2007
Last update date Feb 11, 2019
Contact name zhao chen
E-mail(s) zhao_chen@dfci.harvard.edu
Phone 617-582-7857
Fax 617-582-8490
Organization name Dana Farber Cancer Institute
Street address 1 jimmy fund way
City boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM230945 PlagL2WT1
GSM230946 PlagL2WT2
GSM230947 PlagL2WT3
Relations
BioProject PRJNA102661

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Supplementary file Size Download File type/resource
GSE9123_RAW.tar 25.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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