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Series GSE9124 Query DataSets for GSE9124
Status Public on Nov 01, 2007
Title Gene expression profiling of E12.5 wildtype- and Sp3 null hearts
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mice lacking the zinc finger transcription factor Specificity protein 3 (Sp3) die prenatally in the C57Bl/6 background. To elucidate the cause of mortality we analyzed the potential role of Sp3 in embryonic heart development. Sp3 null hearts display defective looping at E10.5, and at E14.5 the Sp3 null mutants have developed a range of severe cardiac malformations. In an attempt to position Sp3 in the cardiac developmental hierarchy, we analysed the expression patterns of >15 marker genes in Sp3 null hearts. Expression of Cardiac ankyrin repeat protein (Carp) was downregulated prematurely after E12.5, while expression of the other marker genes was not affected. ChIP analysis revealed that Sp3 is bound to the Carp promoter region in vivo. Microarray analysis indicates that small molecule metabolism and cell-cell interactions are the most significantly affected biological processes in E12.5 Sp3 null myocardium. Since the epicardium showed distension from the myocardium, we studied expression of Wt1, a marker for epicardial cells. Wt1 expression was diminished in epicardium-derived cells in the myocardium of Sp3 null hearts. We conclude that Sp3 is required for normal cardiac development, and suggest that it has a crucial role in myocardial differentiation. (
Keywords: Transcription factors, Sp3, knockout mice, cardiac malformations, E12.5
 
Overall design Hearts were dissected from E12.5 wildtype (n=3) and Sp3 knockout (n=3) fetuses. Total RNA was isolated from individual hearts; 5μg was used for labelling and hybridization to 430 2.0 Gene Chips (a total of 6).
 
Contributor(s) van Loo P, Mahtab E, Wisse L, Hou J, Grosveld F, Suske G, Philipsen S, Gittenberger-de Groot A
Citation(s) 17923686
Submission date Sep 21, 2007
Last update date Feb 11, 2019
Contact name Sjaak Philipsen
E-mail(s) j.philipsen@erasmusmc.nl
Phone +31-10-7044282
Fax +31-10-7044743
URL http://www.erasmusmc.nl/medical_genetics/
Organization name Erasmus MC
Department Cell Biology
Street address Dr. Molewaterplein 50
City Rotterdam
ZIP/Postal code 3015 GE
Country Netherlands
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM230954 E12.5 heart_Sp3 knockout_sample a
GSM230955 E12.5 heart_Sp3 knockout_sample b
GSM230956 E12.5 heart_Sp3 knockout_sample c
Relations
BioProject PRJNA102663

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9124_RAW.tar 22.5 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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