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| Status |
Public on Dec 04, 2007 |
| Title |
Identification of genes responding to the activity of the Arabidopsis cytochrome P450 KLUH/CYP78A5 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The Arabidopsis cytochrome P450 KLUH (KLU)/CYP78A5 promotes organ growth in a non-cell autonomous manner. To identify genes regulated by KLU activity, homozygous klu-2 mutants carrying constructs for EtOH-inducible overexpression of wild-type KLU (35S::AlcR-AlcA::KLU) or of enzymatically inactive KLU protein (35S::AlcR-AlcA::KLUmut) were induced with EtOH and sampled at 90 min and 240 min after induction for gene expression changes.
Keywords: Time course, genetic modification
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| Overall design |
For both constructs (i.e. overexpression of wild-type and of mutant KLU protein as a control), two independently transformed lines were used, serving as biological replicates. These were sampled at 90 min and 240 min after induction. In addition, plants with the wild-type construct were also sampled immediately before induction as a control, together resulting in ten hybridizations.
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| Contributor(s) |
Anastasiou E, Kenz S, MacLean D, Lenhard M |
| Citation(s) |
18061566 |
| Submission date |
Oct 01, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Michael Lenhard |
| E-mail(s) |
michael.lenhard@bbsrc.ac.uk
|
| Phone |
0044-1603-450524
|
| Fax |
0044-1603-450025
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| Organization name |
John Innes Centre
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| Department |
Cell & Developmental Biology
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| Street address |
Colney Lane
|
| City |
Norwich |
| State/province |
Norfolk |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA102781 |
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