|
| Status |
Public on May 01, 2018 |
| Title |
Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Skeletal muscle harbors quiescent stem cells termed satellite cells and proliferative progenitors termed myoblasts, which play pivotal roles during muscle regeneration. However, current technology does not allow permanent capture of these cell populations in vitro. Here, we show that ectopic expression of the myogenic transcription factor MyoD, combined with exposure to small molecules, reprograms mouse fibroblasts into expandable induced myogenic progenitor cells (iMPCs). iMPCs express key skeletal muscle stem and progenitor cell markers including Pax7 and Myf5 and give rise to Dystrophin-expressing myofibers upon transplantation, a subset of which maintain Pax7 expression in vivo and sustain serial regenerative responses. Similar to satellite cells, iMPCs originate from Pax7+ cells and require Pax7 itself for maintenance. Finally, we show that iMPCs can be established from muscle tissue following small molecule exposure alone. This study thus reports on a robust approach to derive expandable myogenic stem/progenitor-like cells from multiple differentiated cell types.
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| Overall design |
5 samples were analyzed in total: MEFs, MEFs+MyoD (day 14 of reprogramming), MEFs+MyoD+F/R (day 14 of reprogramming), iMPC clone and C2C12 myoblasts.
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| |
|
| Contributor(s) |
Bar-Nur O, Hochedlinger K |
| Citation(s) |
29742392 |
| Submission date |
Dec 13, 2016 |
| Last update date |
Jul 31, 2018 |
| Contact name |
Ori Bar-Nur |
| E-mail(s) |
ori.bar-nur@mail.huji.ac.il
|
| Organization name |
Harvard University
|
| Department |
Center of Regenerative medicine
|
| Lab |
Hochedlinger
|
| Street address |
185 Cambridge st.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (5)
|
| GSM2427778 |
Mouse embryonic fibroblasts |
| GSM2427779 |
Mouse embryonic fibroblasts (sorted for Thy1) induced to express MyoD for 14 days, sorted for the MEF marker Thy1 |
| GSM2427780 |
Mouse embryonic fibroblasts (sorted for Thy1) induced to express MyoD in the presence of small moleculesfor 14 days, |
|
| Relations |
| BioProject |
PRJNA357260 |
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