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| Status |
Public on Mar 18, 2017 |
| Title |
Ectopic Drosophila AKH overexpression effect on gene expression in the 3rd instar larval fat body |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of the larval fat bodies overexpressing AKH, which activates AKH signaling in the fat body in an autocrine manner. As a homolog of human glucagon, AKH causes dysregulation of carbohydrate metabolism and hyperglycemia. Results provide insight into the AKH effects on metabolic gene expression.
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| Overall design |
The fat bodies were dissected from late 3rd instar Drosophila larvae of control (R4-Gal4/+) and AKH-overexpressing (UAS-AKH/+; R4-Gal4/+) strains.RNAs were extracted for deep sequencing, in triplicate, using Illumina Hiseq 2000.
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| Contributor(s) |
Song W, Cheng D, Perrimon N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Wei Song |
| E-mail(s) |
rocwsong@gmail.com, songw@whu.edu.cn
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| Organization name |
Harvard Medical School
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| Department |
Department of Genetics
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| Street address |
77 Ave Louis Pasteur, NRB 336
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA357276 |
| SRA |
SRP095043 |
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