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Series GSE9244 Query DataSets for GSE9244
Status Public on Dec 31, 2008
Title WT vs Klf5 KO ES
Organism Mus musculus
Experiment type Expression profiling by array
Summary Homozygous disruption of Bteb2/Klf5, a homolog of Drosophila gap gene Kr├╝ppel, led to increased expression of various differentiation marker genes, such as Fgf5, Cdx2, and Brachyury in mouse ES cells without compromising their ability to differentiate into all three germ layers. Upon removal of LIF, Klf5-deficient ES cells showed faster differentiation kinetics than wild-type ES cells. In contrast, overexpression of Klf5 in ES cells suppressed the transcription of differentiation marker genes, and maintained pluripotency in the absence of LIF. In order to search downstream genes of Klf5, we surveyed genes implicated in ES cell proliferation by microarray analysis
Keywords: cell type comparison
 
Overall design WT (clone 1 and 2) and Klf5 KO (clone 1 and 2) ES cells were maintained in ES medium + 15%FBS + LIF. Total RNAs from those ES cells were prepared using RNeasy Kit. Genes down-regulated in Klf5 KO ES cells were searched by GeneSpring software.
 
Contributor(s) Uchida K, Ema M
Citation(s) 18983969
Submission date Oct 05, 2007
Last update date Feb 11, 2019
Contact name Kazuhiko Uchida
E-mail(s) kazuhiko.uchida@cbiri.org
Phone 81-29-861-3000
Fax 81-29-855-5271
Organization name University of Tsukuba
Department Institute of Basic Medical Sciences
Lab Molecular and biological Oncology
Street address 1-1-1 Tennnoudai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8575
Country Japan
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM234772 Mouse_WT_ES_clone 1
GSM234773 Mouse_WT_ES_clone 2
GSM234774 Mouse_Klf5 KO_ES_clone 1
Relations
BioProject PRJNA102853

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9244_RAW.tar 15.0 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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