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| Status |
Public on Oct 09, 2007 |
| Title |
Kaposi's Sarcoma-associated Herpesvirus Encodes an Ortholog of miR-155 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs are small, non-coding RNAs that post-transcriptionally regulate gene expression by binding to 3’UTRs of target mRNAs. Kaposi’s sarcoma-associated herpesvirus (KSHV), a virus linked to malignancies including primary effusion lymphoma (PEL), encodes 12 miRNA genes, but only a few regulatory targets are known. We found that KSHV-miR-K12-11 shares 100% seed-sequence homology with hsa-miR-155, a miRNA frequently found up-regulated in lymphomas and critically important for B cell development. Based on this seed-sequence homology, we hypothesized that both miRNAs regulate a common set of target genes and as a result, could have similar biological activities.
Examination of five PEL lines showed that PELs do not express miR-155, but do express high levels of miR-K12-11. Bioinformatics tools predicted the transcriptional repressor BACH-1 to be targeted by both miRNAs and ectopic expression of either miR-155 or miR-K12-11 inhibited a BACH-1 3'UTR containing reporter. . Furthermore, BACH-1 protein levels are low in cells expressing either miRNA. Gene expression profiling of miRNA-expressing stable cell lines revealed 66 genes that were commonly down-regulated. For select genes, miRNA targeting was confirmed by reporter assays.
Thus, based on our in silico predictions, reporter assays, and expression profiling data, miR-K12-11 and miR-155 regulate a common set of cellular targets. Given the role of miR-155 during B cell maturation, we speculate that miR-K12-11 may contribute to the distinct developmental phenotype of PEL cells, which are blocked in a late stage of B cell development. Together, these findings indicate that KSHV miR-K12-11 is an ortholog of miR-155.
Keywords: comparison, experiemental versus control
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| Overall design |
12 samples, 4 experiemental (miR-155 ), 4 experimental (miR-K12-11) and 4 reference controls (pCDNA3.1)
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| Contributor(s) |
Skalsky RL, Samols MA, Plaisance KB, Boss IW, Riva A, Lopez MC, Baker HV, Renne R |
| Citation(s) |
17881434 |
| Submission date |
Oct 09, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
M Cecilia Lopez |
| E-mail(s) |
mclopez@ufl.edu
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| Phone |
352 273-8133
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| Organization name |
University of Florida
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| Department |
Molecular Genetics and Microbiology
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| Lab |
Microarray lab 103
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| Street address |
1376 Mowry Road
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610-3610 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA102893 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9264_RAW.tar |
94.7 Mb |
(http)(custom) |
TAR (of CEL, EXP) |
| Processed data included within Sample table |
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