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Series GSE92690 Query DataSets for GSE92690
Status Public on Dec 22, 2016
Title Transcriptome profiling of P-granule-depleted or P-granule mutant gonads versus control gonads over time
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary The germ cells of multicellular organisms protect their developmental potential through specialized mechanisms. A shared feature of germ cells from worms to humans is the presence of non-membrane-bound ribonucleoprotein organelles called germ granules. Depletion of germ granules in Caenorhabditis elegans (i.e., P granules) leads to sterility and in some germlines expression of the neuronal transgene unc-119::gfp and the muscle myosin MYO-3. Thus, P granules are hypothesized to maintain germ cell totipotency by preventing somatic development, although the mechanism by which P granules carry out this function is unknown. In this study, we performed transcriptome and single molecule RNA-FISH analyses of dissected P-granule-depleted gonads at different developmental stages. Our results demonstrate that P granules are necessary for adult germ cells to down-regulate spermatogenesis RNAs and to prevent the accumulation of numerous soma-specific RNAs. P-granule-depleted gonads that express the unc-119::gfp transgene also express many other genes involved in neuronal development and concomitantly lose expression of germ cell fate markers. Finally, we show that removal of either of two critical P-granule components, PGL-1 or GLH-1, is sufficient to cause germ cells to express UNC-119::GFP and MYO-3 and to display RNA accumulation defects similar to those observed after depletion of P granules. Our data identify P granules as critical modulators of the germline transcriptome and guardians of germ cell fate.
 
Overall design Transcriptome profiles of 3 replicates of L4 P-granule RNAi gonads, 3 replicates of L4 control gonads, 3 replicates of Day 1 Adult P-granule RNAi gonads grown at 24°C
 
Contributor(s) Knutson AK, Egelhofer T, Rechtsteiner A, Strome S
Citation(s) 28258184
Submission date Dec 21, 2016
Last update date May 15, 2019
Contact name Susan Strome
E-mail(s) sstrome@ucsc.edu
Organization name UC Santa Cruz
Department MCD Biology
Street address 1156 High St
City Santa Cruz
State/province CA
ZIP/Postal code 95064
Country USA
 
Platforms (3)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
GPL22765 Illumina HiSeq 4000 (Caenorhabditis elegans)
Samples (29)
GSM2435377 L4 larval control gonads #1
GSM2435378 L4 larval control gonads #2
GSM2435379 L4 larval control gonads #3
Relations
BioProject PRJNA358377
SRA SRP095453

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92690_File.S1.processed.transcriptome.data_one.sheet.xlsx 15.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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