|
| Status |
Public on Jan 06, 2017 |
| Title |
miRNA data in human placentas from normal pregnancies and from pregnancies complicated by SGA and IUGR |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Placental insufficiency leading to intrauterine growth restriction demonstrates perturbed gene expression affecting placental angiogenesis and nutrient transfer from mother to fetus. To understand the post-transcriptional mechanisms underlying such placental gene expression changes, our objective was to identify key non-coding microRNAs that express biological function. To this end, we undertook microarray targeting microRNAs in placentas of appropriate (AGA, n=3) versus small for gestational age (SGA, n=3) weight infants and observed upregulation of 97 miRs in SGA versus AGA. In a larger cohort of samples, we validated by qRT-PCR, differential expression of three specific microRNAs that target genes mediating angiogenesis and nutrient transfer.
We performed microarray on a small cohort of samples in order to identify differential expression in pathways involved with angiogenesis and nutrient transfer, essential processes for placental function that when perturbed, may result in intrauterine growth restriction.
|
| |
|
| Overall design |
Human placental samples were obtained immediately post-parturient. There were three samples from the AGA group (Bw>10% and <90%) and three samples from the SGA/IUGR (Birthweight <10%) group. All placental samples were from term, singleton pregnancies.
|
| |
|
| Contributor(s) |
Chu A, Devaskar SU |
| Citation(s) |
28463968 |
| Submission date |
Jan 05, 2017 |
| Last update date |
Jul 27, 2018 |
| Contact name |
Alison Chu |
| E-mail(s) |
AlisonChu@mednet.ucla.edu
|
| Organization name |
UCLA
|
| Street address |
10833 LeConte Avenue
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
|
| Samples (6)
|
| GSM2445874 |
human term placenta from AGA pregnancy C1 |
| GSM2445875 |
human term placenta from AGA pregnancy C14 |
| GSM2445876 |
human term placenta from AGA pregnancy C7 |
| GSM2445877 |
human term placenta from IUGR pregnancy U2 |
| GSM2445878 |
human term placenta from IUGR pregnancy U4 |
| GSM2445879 |
human term placenta from IUGR pregnancy U6 |
|
| Relations |
| BioProject |
PRJNA360238 |
Less...
More...