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| Status |
Public on Oct 07, 2017 |
| Title |
Architectural alterations of the fission yeast genome across the cell cycle |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Eukaryotic genomes are highly ordered through various mechanisms, including topologically associating domain (TAD) organization. We employed an in situ Hi-C approach to follow the 3D organization of the fission yeast genome during the cell cycle. We demonstrate that during mitosis, large domains of 300 kb–1 Mb are formed by condensin. This mitotic domain
organization does not suddenly dissolve, but gradually diminishes until the next mitosis. By contrast, small domains of 30–40 kb that are formed by cohesin are relatively stable across the cell cycle. Condensin and cohesin mediate long- and short-range contacts, respectively, by bridging their binding sites, thereby forming the large and small domains. These domains are inversely regulated during the cell cycle but assemble independently. Our study describes the chromosomal oscillation between the formation and decay phases of the large and small domains, and we predict that the condensin-mediated domains serve as chromosomal compaction units.
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| Overall design |
Hi-C experiment of 20,30,40,50,60,70,80,120min and cut3, cut14, rad21, cut14 & cut3 double mutant and wild type cell. RNA-seq experiment of wild type, cut14, cut3 mutant.
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| Contributor(s) |
Tanizawa H, Noma K |
| Citation(s) |
28991264 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM124195 |
Condensin connects 3D genome organization, chromosomal segregation, and gene regulation via its interactions with transcription-related factors |
THE WISTAR INSTITUTE |
Kenichi Noma |
| DP2 OD004348 |
A New Methodology to Decipher Three-Dimensional Genome Structure |
THE WISTAR INSTITUTE |
Kenichi Noma |
| P30 CA010815 |
Consolidated Basic Cancer Research Program |
THE WISTAR INSTITUTE |
Dario C Altieri |
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| Submission date |
Jan 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hideki Tanizawa |
| E-mail(s) |
hidekit@uoregon.edu
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| Organization name |
University of Oregon
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| Department |
Institute of Molecular Biology
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| Street address |
1370 Franklin Blvd
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| City |
Eugene |
| State/province |
OR |
| ZIP/Postal code |
97403 |
| Country |
USA |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA360306 |
| SRA |
SRP096140 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE93198_RAW.tar |
819.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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