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| Status |
Public on Jan 09, 2017 |
| Title |
RNA sequencing of organoids isolated from pancreatic cancer mouse model KRASG12D; p53R172H; Ptf1a-Cre. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We studied the transcriptomics of pancreatic ductal adenocarcinoma (PDAC) and the role of a splicing factor called SF3B1 in this context. We generated mice that will develop PDAC via an inducible expression of KRASG12D; p53R172H specifically in the pancreas using a Ptf1a-Cre.
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| Overall design |
We have isolated organoids from:
KrasG12D; p53R172H (KP),
KrasG12D; p53R172H; Ptf1a-Cre (KPC) ,
KrasG12D; p53R172H; SF3b1 Fl/+ (KPS),
and KrasG12D; p53R172H; Ptf1a-Cre; SF3b1 Fl/+ (KPCS) mice.
We have 3 organoids per group, representing 3 biological replicates. We have exposed the organoids to normoxia (21% O2) or hypoxia (3%O2).
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| Contributor(s) |
Cortijo C, Krek W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Cédric Cortijo |
| E-mail(s) |
cedric.cortijo@biol.ethz.ch
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| Organization name |
ETHZ
|
| Department |
HPL
|
| Street address |
Otto-Stern-Weg 7
|
| City |
zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA360459 |
| SRA |
SRP096208 |
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