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| Status |
Public on Jan 11, 2018 |
| Title |
Comparison of gene expression profilings between Mock-WS480, NonV50-OKS-SW480 and 2ndV50-OKS-SW480 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have previously reported that colon cancer stem-like cells can be induced from human colon cancer cell lines by retrovirally introducing OCT3/4, SOX2 and KLF4, and we designated these cells as induced cancer stem cells (iCSCs). iCSCs showed significantly enhanced CSC properties in terms of the marker gene expression, sphere formation, chemoresistance and tumorigenicity. In the current study, we generated iCSCs using a polycistronic retroviral expression vector, and indicated that iCSCs-derived tissues in vitro are able to recapitulate human colon cancer tissues. To identify the molecular mechanisms that promote the properties of CSCs, we compared the global gene expression patterns of Mock-SW480 cells, non-V50 cells from 1st V50-OKS cells, and 2nd V50-OKS cells.
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| Overall design |
We extracted total RNA from 3 Mock-SW480 cells, 3 NonV50-cells from 1st V50-OKS cells and 3 2ndV50-OKS cells and hybridized them to Agilent gene expression microarrays.
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| Contributor(s) |
Ishida R |
| Citation(s) |
28710230 |
| Submission date |
Jan 11, 2017 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Michiyo Koyanagi-Aoi |
| E-mail(s) |
mkoyaoi@med.kobe-u.ac.jp
|
| Organization name |
Kobe University
|
| Street address |
Chuo-ku, Kusunoki-cho 7-5-1
|
| City |
Kobe City |
| ZIP/Postal code |
650-0017 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA360906 |
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