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Status |
Public on Jan 11, 2020 |
Title |
Xenophagic process controls the Enteroinvasive Escherichia coli infection of the epithelial cells II |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The invasive bacteria recognition by host cells through autophagy is a key factor for determining bacterial infection. Enteroinvasive Escherichia coli (EIEC) express a protein IcsB, which in Shigella, is known for inactivating the bacterial degradation process. Once EIEC showed less expression of icsB when compared to S. flexneri, we proposed to investigate the autophagy caused by EIEC infection. Our results showed that IcsB protein is an important virulence factor in EIEC because it causes a camouflage of the bacteria in the eukaryotic cell. When there is low or none expression of the protein, the cell recognition of the invasive bacteria is high, decreasing the bacteria dissemination. This found confirms the importance of the gene transcription and the sequence, since the strain E. coli SM124/13, complemented with icsB from Shigella, showed higher dissemination efficiency inside of the host cell. Additionally, our results revealed that eukaryotic cell infected by EIEC or Shigella flexneri showed distinguish responses. In EIEC infection, the autophagy was activated in human cells, but not in a conventional mode. Our hypothesis is that EIEC is recognized by autophagy, being an important cell process for bacterial recognition.
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Overall design |
Caco-2 transcriptomic profiles with and without bacterial infections were compared in order to identify differentially expressed transcripts at different interaction periods (up to 3 hrs).
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Contributor(s) |
Santos HC, Ramos RM, Ferreira LG, Bando SY, Bertonha FB, Ferreira LR, Moreira-Filho CA, Abe CM, Moreno AR, Martinez MB |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Jan 13, 2017 |
Last update date |
Jan 13, 2020 |
Contact name |
Hadassa Cristhina Azevedo Soares Santos |
E-mail(s) |
hadassa.cass@gmail.com
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Phone |
55 11 959921111
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Organization name |
University of São Paulo
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Department |
Department of Clinical Chemistry and Toxicology
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Lab |
Lab of Microbiology
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Street address |
Avenida Professor Lineu Prestes, 580
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City |
São Paulo |
State/province |
São Paulo |
ZIP/Postal code |
05508-900 |
Country |
Brazil |
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Platforms (1) |
GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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Samples (16)
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GSM2455348 |
Caco-2 cell without interaction - control (0h), replicate 1 |
GSM2455349 |
Caco-2 cell without interaction - control (0h), replicate 2 |
GSM2455350 |
Caco-2 cell without interaction - control (0h), replicate 3 |
GSM2455351 |
Caco-2 cell without interaction - control (0h), replicate 4 |
GSM2455352 |
Caco-2 cell without interaction - control (1h), replicate 1 |
GSM2455353 |
Caco-2 cell without interaction - control (1h), replicate 2 |
GSM2455354 |
Caco-2 cell without interaction - control (1h), replicate 3 |
GSM2455355 |
Caco-2 cell without interaction - control (1h), replicate 4 |
GSM2455356 |
Caco-2 cell without interaction - control (2h), replicate 1 |
GSM2455357 |
Caco-2 cell without interaction - control (2h), replicate 2 |
GSM2455358 |
Caco-2 cell without interaction - control (2h), replicate 3 |
GSM2455359 |
Caco-2 cell without interaction - control (2h), replicate 4 |
GSM2455360 |
Caco-2 cell without interaction - control (3h), replicate 1 |
GSM2455361 |
Caco-2 cell without interaction - control (3h), replicate 2 |
GSM2455362 |
Caco-2 cell without interaction - control (3h), replicate 3 |
GSM2455363 |
Caco-2 cell without interaction - control (3h), replicate 4 |
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Relations |
BioProject |
PRJNA361229 |
Supplementary file |
Size |
Download |
File type/resource |
GSE93587_RAW.tar |
145.2 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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