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Series GSE93587 Query DataSets for GSE93587
Status Public on Jan 11, 2020
Title Xenophagic process controls the Enteroinvasive Escherichia coli infection of the epithelial cells II
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The invasive bacteria recognition by host cells through autophagy is a key factor for determining bacterial infection. Enteroinvasive Escherichia coli (EIEC) express a protein IcsB, which in Shigella, is known for inactivating the bacterial degradation process. Once EIEC showed less expression of icsB when compared to S. flexneri, we proposed to investigate the autophagy caused by EIEC infection. Our results showed that IcsB protein is an important virulence factor in EIEC because it causes a camouflage of the bacteria in the eukaryotic cell. When there is low or none expression of the protein, the cell recognition of the invasive bacteria is high, decreasing the bacteria dissemination. This found confirms the importance of the gene transcription and the sequence, since the strain E. coli SM124/13, complemented with icsB from Shigella, showed higher dissemination efficiency inside of the host cell. Additionally, our results revealed that eukaryotic cell infected by EIEC or Shigella flexneri showed distinguish responses. In EIEC infection, the autophagy was activated in human cells, but not in a conventional mode. Our hypothesis is that EIEC is recognized by autophagy, being an important cell process for bacterial recognition.
 
Overall design Caco-2 transcriptomic profiles with and without bacterial infections were compared in order to identify differentially expressed transcripts at different interaction periods (up to 3 hrs).
 
Contributor(s) Santos HC, Ramos RM, Ferreira LG, Bando SY, Bertonha FB, Ferreira LR, Moreira-Filho CA, Abe CM, Moreno AR, Martinez MB
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Submission date Jan 13, 2017
Last update date Jan 13, 2020
Contact name Hadassa Cristhina Azevedo Soares Santos
E-mail(s) hadassa.cass@gmail.com
Phone 55 11 959921111
Organization name University of São Paulo
Department Department of Clinical Chemistry and Toxicology
Lab Lab of Microbiology
Street address Avenida Professor Lineu Prestes, 580
City São Paulo
State/province São Paulo
ZIP/Postal code 05508-900
Country Brazil
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (16)
GSM2455348 Caco-2 cell without interaction - control (0h), replicate 1
GSM2455349 Caco-2 cell without interaction - control (0h), replicate 2
GSM2455350 Caco-2 cell without interaction - control (0h), replicate 3
Relations
BioProject PRJNA361229

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93587_RAW.tar 145.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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