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| Status |
Public on Jan 19, 2018 |
| Title |
Comparison of HBO1 siRNA-treated Human Umbilical Vein Endothelial Cells (HUVECs) and control siRNA-treated HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
To determine the role of HBO1 in EC physiology, gene expression analysis was conducted on control and HBO1 siRNA-treated HUVECs. A total of 263 differentially regulated protein-coding transcripts were detected, many of which are key for growth and angiogenesis. Additionally, many genes involved in cell cycle, cell division, and DNA replication were dysregulated. HBO1-regulated genes were verified by qRT-PCR, including those with roles in vessel tone regulation (e.g. ACE1), vessel formation (e.g. CXCL16), EC activation (e.g. E-selectin), and cholesterol efflux (e.g. ABCG1).
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| Overall design |
HUVECs were treated with control siRNA or HBO1 siRNA for 72 hours. Subsequently, these samples were subjected to gene expression analysis using a custom agilent microarray that recognizes 26109 protein-coding transcripts and 30586 lncRNAs.
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| Contributor(s) |
Marsden PA, Yan MS |
| Citation(s) |
29414790 |
| Submission date |
Jan 13, 2017 |
| Last update date |
Apr 24, 2018 |
| Contact name |
Philip A Marsden |
| E-mail(s) |
p.marsden@utoronto.ca
|
| Organization name |
University of Toronto
|
| Department |
Medicine
|
| Street address |
209 Victoria Street, Room 522
|
| City |
Toronto |
| ZIP/Postal code |
M5B 1C6 |
| Country |
Canada |
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| Platforms (1) |
| GPL16956 |
Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA361276 |
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