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Series GSE93625 Query DataSets for GSE93625
Status Public on Sep 22, 2017
Title Human colon cancer SW480 cells: stably transfected with vector control and LRP16 treated with or without etoposide
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Transcriptional profiling of human colon cancer SW480 cells comparing control untreated SW480 cells with cells stably transfected with LRP16 treated with or without etoposide (50 μM) for the indicated periods (0,1hour, 3hours).An exploratory microarray analysis was performed with mRNA extracted from clutured SW480 cells transfected with LRP16 or control plasmid that were treated with or without etoposide. Total RNA of colon cancer cells stably transfected with vector control and LRP16 treated with or without etoposide (50 μM) for the indicated periods was isolated and purified using RNeasy Kit (Qiagen, Hilden, Germany). Integrity of RNA was assessed by using an Agilent BioAnalyser 2100 (Agilent Technologies).
 
Overall design An exploratory microarray analysis was performed with mRNA extracted from clutured SW480 cells transfected with LRP16 or control plasmid that were treated with or without etoposide. Three independent experiments were performed at each time (0, 1 or 3 hours) using different group cells for each experiment.
 
Contributor(s) Li X, Wu Z, Han W
Citation(s) 28820388
Submission date Jan 13, 2017
Last update date Mar 12, 2018
Contact name Xiaolei Li
E-mail(s) li-xiaolei0913@163.com
Phone 86(10)66937519
Organization name Institute of Basic Medicine, Chinese PLA General Hospita
Street address #28 Fuxing Road,
City Beijing
ZIP/Postal code 100853
Country China
 
Platforms (1)
GPL16956 Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version)
Samples (18)
GSM2459077 SW480 cells_Stably transfected with pc3.1 untreated1#
GSM2459078 SW480 cells_Stably transfected with pc3.1 untreated 2#
GSM2459079 SW480 cells_Stably transfected with pc3.1 untreated 3#
Relations
BioProject PRJNA361306

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93625_RAW.tar 49.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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