|
| Status |
Public on May 22, 2017 |
| Title |
Transcriptional comparisons of stem cell-derived hepatocytes (hiPS-Hep), HepaRG cells and 3D human hepatocyte spheroids as predictive in vitro systems for drug-induced liver injury |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Three current hepatic in vitro cell systems were compared at the gene expression level in order to determine their suitability for investigations of hepatotoxicity. For mechanistic investigations we focussed on the PHH spheroid system, treating them with IC10 concentrations of either aflatoxin B1, amiodarone or chlorpromazine for up to 2 weeks and investigating the transcriptional changes induced by each of the compounds. We observed significant alterations in genes regulating carcinogenic, steatotic and cholestatic pathologies respectively.
|
| |
|
| Overall design |
At 48h, 7 days (3 treatments) and 14 days (6 treatments) cells were harvested and RNA isolated. For each treatment three technical replicates were analysed.
|
| |
|
| Contributor(s) |
Bell C, Lauschke V, Ingelman-Sundberg M |
| Citation(s) |
28137721 |
| Submission date |
Jan 19, 2017 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Magnus Ingelman-Sundberg |
| E-mail(s) |
Magnus.Ingelman-Sundberg@ki.se
|
| Organization name |
Karolinska Institutet
|
| Street address |
Nanna Svartz väg 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (54)
|
|
| Relations |
| BioProject |
PRJNA362580 |
Less...
More...