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Series GSE93898 Query DataSets for GSE93898
Status Public on Feb 18, 2017
Title MMP28 is a key contributor to emphysema pathogenesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Chronic Obstructive Pulmonary Disease, which is comprised of chronic bronchitis and emphysema, is a leading cause of morbidity and mortality. Because tissue destruction is the prominent characteristic of emphysema, extracellular proteinases, particularly those with elastolytic ability, are often considered to be key drivers in this disease. Several human and mouse studies have implicated roles for matrix metalloproteinases (MMPs), particularly macrophage-derived proteinases, in COPD pathogenesis. MMP28 is expressed by the pulmonary epithelium and macrophage, and we have found that it regulates macrophage recruitment and polarization. We hypothesized that MMP28 would have contributory roles in emphysema via alteration of macrophage numbers and activation. Because of the established association of emphysema pathogenesis to macrophage influx, we evaluated the inflammatory changes and lung histology of Mmp28-/- mice exposed to 3 and 6 months of cigarette smoke. At earlier time-points, we found altered macrophage polarization in the smoke-exposed Mmp28-/- lung consistent with other published findings that MMP28 regulates macrophage activation. At both 3 and 6 months, Mmp28-/- mice had blunted inflammatory responses more closely resembling non-smoked mice, with a reduction in neutrophil recruitment and CXCL1 chemokine expression. By 6 months, Mmp28-/- mice were protected from emphysema. These results highlight a previously unrecognized role for MMP28 in promoting chronic lung inflammation and tissue remodeling induced by cigarette smoke and highlight another potential target to modulate COPD.
Overall design Total RNA from whole lungs homogenates of wildtype and knockout (Mmp28-/-) mice exposed to room air or 3 months of cigarette smoke was isolated and hybidized to Illumina Mouse Ref-8 v2 Expression BeadChips.
Contributor(s) Gharib SA
Citation(s) 28399390
Submission date Jan 21, 2017
Last update date Jun 14, 2018
Contact name Sina A Gharib
Phone 206-221-0630
Organization name University of Washington
Department Medicine
Street address 850 Republican
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM2464602 wild type exposed to cigarette smoke replicate 1
GSM2464603 wild type exposed to cigarette smoke replicate 2
GSM2464604 wild type exposed to cigarette smoke replicate 3
BioProject PRJNA362783

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE93898_RAW.tar 3.1 Mb (http)(custom) TAR
GSE93898_non-normalized.txt.gz 2.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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