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| Status |
Public on Jul 23, 2017 |
| Title |
Distinctive transcriptome alterations of prefrontal pyramidal neurons in schizophrenia and schizoaffective disorder |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Schizophrenia is associated with alterations in working memory that reflect dysfunction of dorsolateral prefrontal cortex (DLPFC) circuitry. Working memory depends on the activity of excitatory pyramidal cells in DLPFC layer 3, and to a lesser extent in layer 5.
Although many studies have profiled gene expression in DLPFC gray matter in schizophrenia, little is known about cell type-specific transcript expression in these two populations of pyramidal cells. We hypothesized that interrogating gene expression specifically in DLPFC layer 3 or 5 pyramidal cells would reveal new and/or more robust schizophrenia-associated differences that would provide new insights into the nature of pyramidal cell dysfunction in the illness.
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| Overall design |
Individual pyramidal cells in DLPFC layers 3 or 5 were captured by laser microdissection from 36 subjects with schizophrenia or schizoaffective disorder and matched normal comparison subjects.
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| Contributor(s) |
Arion D, Corradi JP, Tang S, Datta D, Boothe F, He A, Cacace AM, Zaczek R, Albright CF, Tseng G, Lewis DA |
| Citation(s) |
25560755 |
| Submission date |
Jan 24, 2017 |
| Last update date |
Apr 20, 2018 |
| Contact name |
David Lewis |
| E-mail(s) |
lewisda@upmc.edu
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| Organization name |
University of Pittsburgh
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| Department |
Psychiatry and Neuroscience
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| Street address |
3811 O'Hara St
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (208)
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| Relations |
| BioProject |
PRJNA363012 |
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