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Series GSE94074 Query DataSets for GSE94074
Status Public on Mar 01, 2017
Title Expression data of Hematopoietic progenitor and stem cells after 18h of culture with or without extracellular vesicles secreted by AFT stromal cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hematopoietic progenitor and stem cells from bone marrow have been sorted by FACS (LSK, Lineage -, Sca1 + and cKit +) and co-culture during 18h without cytokines with or without extracellular vesicles (EV) secreted by AFT stromal cells.
We used microarray to detail the modification of gene expression level of LSK cells after contact with AFT Evs
 
Overall design 3 conditions were analysed for gene expression by microarrays. LSK cells after cell sorting, LSK cells cultured 18h without EV and LSK cells cultured 18h with EVs derived from AFT cells. For each conditions 5 biological replicates have been used
 
Contributor(s) Gregoire S, Charles D
Citation(s) 28630143
Submission date Jan 25, 2017
Last update date Nov 09, 2018
Contact name Gregoire Stik
E-mail(s) gregoire.stik@gmail.com
Organization name CRG
Street address 88 Dr Aiguader
City Barcelona
State/province select a state
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (15)
GSM2468274 LSK cultured 18h with EV rep1
GSM2468275 LSK cultured 18h with EV rep2
GSM2468276 LSK cultured 18h with EV rep3
Relations
BioProject PRJNA368725

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94074_RAW.tar 126.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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