|
| Status |
Public on Mar 13, 2017 |
| Title |
Characterization of murine pulmonary interstitial macrophages at steady state |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we demonstrate that the lung mononuclear phagocyte system comprises three interstitial macrophages (IMs), as well as alveolar macrophages (AMs), dendritic cells and few extravascular monocytes.
Through cell sorting and RNAseq analysis we were able to identify transcriptional similarities and differences between the three pulmonary IM subtypes, with reference to the more well-characterized alveolar macrophage
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| |
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| Overall design |
Pulmonary Interstitial and Alveolar macrophages were FACS sorted from the lungs of steady state 8-10 week old B6 mice, in triplicate. Extracted RNA was examined by RNAsequencing.
The tar archive GSE94135_jakubzick_2019*tar available at the foot of this page contains the supplementary processed data used for comparisons with data in GSE132911. Data were processed as described in GSE132911.
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| |
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| Contributor(s) |
Jakubzick CV, Gibbings SL, Danhorn T, Leach SM |
| Citation(s) |
28257233, 33147458 |
| Submission date |
Jan 27, 2017 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Claudia Jakubzick |
| E-mail(s) |
jakubzickc@njhealth.org
|
| Phone |
303-398-1069
|
| Organization name |
National Jewish Health
|
| Department |
Pediatrics
|
| Street address |
1400 Jackson St
|
| City |
Denver |
| State/province |
Colorado |
| ZIP/Postal code |
80206 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
|
| Samples (38)
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| This SubSeries is part of SuperSeries: |
| GSE132912 |
Human and murine transcriptome profiling identifies cross-species homology in pulmonary mononuclear phagocytes |
|
| Relations |
| BioProject |
PRJNA369038 |
| SRA |
SRP098047 |
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