|
| Status |
Public on Feb 18, 2017 |
| Title |
RNAseq analysis of B cells inducible for murine gammaherpesvirus 68 reactivation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RTA, the viral Replication and Transcription Activator, is essential for rhadinovirus lytic gene expression upon de novo infection and reactivation from latency. LPS/TLR4 engagement enhances rhadinovirus reactivation. We developed the HE-RIT cell line, a latent murine A20 B cell inducible for Flag-RTA expression and murine gammaherpesvirus 68 reactivation. LPS acted as a second stimulus to drive virus reactivation from latency in the context of induced expression of FLAG-RTA. We applied RNAseq to examine for genome-wide changes in viral gene expression in response to doxycycline-induced RTA-FLAG, alone or in combination with LPS.
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| |
|
| Overall design |
6 samples total: Replicates of Mock (untreated controls), Dox (induced for RTA expression), and Dox + LPS (dual treatment)
|
| |
|
| Contributor(s) |
Krug LT, Malik L |
| Citation(s) |
28212352 |
| Submission date |
Jan 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Laurie Krug |
| Organization name |
Stony Brook University
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
130 Life Sciences Bldg
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA369112 |
| SRA |
SRP098149 |
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